Project P01

Investigation of the compositional, positional and functional heterogeneity of MICOS clusters in single mammalian cells

Research Group

 

The organization of exo- and endocytotic proteins within a model synaptic bouton.

Sections through the synaptic bouton in which only the exocytotic (A) or the endocytotic proteins (B) are shown. The graphical legend indicates the different proteins (right). The graphical legend indicates the different proteins (bottom). Displayed synaptic vesicles have a diameter of 42 nm.

Principal investigator
Principal investigatorProf. Dr. Stefan Jakobs
University Medical Center Göttingen
Clinic of Neurology
Robert-Koch-Straße 40
D-37075 Göttingen

+49 551 201 2531 (phone)
sjakobs@gwdg.de (e-mail)

jakobs.mpibpc.mpg.de
(research group)

About

The mitochondrial contact site and cristae organizing system (MICOS) is a large multi-subunit complex in the inner membrane of mitochondria that is required for the maintenance of crista junctions.

The MICOS complex is an interaction hub that connects the mitochondrial inner membrane to outer membrane proteins and presumably also to the endoplasmic reticulum (ER).

The MICOS clusters exhibit a well-ordered arrangement in the mitochondria. We will investigate to what extent mitochondrial movements as well as fission and fusion dynamics and interactions with cellular structures such as the ER influence the inner-mitochondrial distribution of MICOS. Thereby this project aims to investigate the compositional and functional heterogeneity of MICOS clusters with respect to their sub-mitochondrial localizations using optical nanoscopy and electron microscopy.