Project P15

Mechanisms of substrate selection in ER associated protein degradation

Principal investigator
Principal investigatorDr. Alexander Stein
Max Planck Institute for
Multidisciplinary Sciences
Am Faßberg 11
D-37077 Göttingen

+49 551 201 1621 (phone) (e-mail)
(research group)


Misfolded proteins of the endoplasmic reticulum (ER) are moved across the ER membrane into the cytosol where they are poly-ubiquitinated and degraded by the proteasome. This process is called ER associated protein degradation (ERAD) or retrotranslocation. The mechanism of ERAD is poorly understood. In this project, we want to answer the questions of how proteins are selected for retrotranslocation and how retrotranslocation is regulated. To this end, we will combine in vitro reconstitution experiments with purified proteins and experiments in intact cells using the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe as model organisms.